GAR-B2 Protocol    

Protocol for use of GAR-B2 reagent for detection of Gaussia Luciferase:

The GAR-B2 reagent gives the brightest bioluminescent signal for both Gaussia and renilla luciferase assays

Dilute 100X coelenterazine substrate with assay dilution buffer supplied with each product just before use. 

Assay 20 µl of the gaussia luciferase sample with 50 µl Gaussia luciferase assay reagent (GAR-B2). If activity is too high dilute with serum-free DMEM but use 20 µl total volume per assay using 50 µl of Gaussia luciferase assay reagent.  This assay reagent is compatible with measuring renilla and Gaussia luciferase activities in cells lysed with either lysis buffers from Targeting Systems or the Promega passive lysis buffer.

Mix well and read in the luminometer.