GAR-B2 Protocol    

Protocol for use of GAR-B2 reagent for detection of Gaussia Luciferase:

Dilute 100X coelenterazine substrate with assay dilution buffer supplied with each product just before use. 

Add 8 µl of stabilizer to 20 µl of Gaussia luciferase samples, mix well and add 50 µl of Gaussia luciferase assay reagent, mix well and read in the luminometer.

Catalog #0090A: Protocol #1 Luminescent signal is stable for 15 minutes, for this version add stabilizer directly to the buffer at a ratio of 8 µl stabilizer to 50 µl Gar-2 buffer. Dilute 100X coelenterazine substrate (marked #0090A) with buffer to 1X. Assay 20 µl Gaussia luciferase sample with 20 µl complete buffer.