GAR-1 Protocol    

Protocol for use of GAR-1 reagent for detection of Gaussia Luciferase:

          Dilute 100X coelenterazine substrate with assay dilution

          buffer  supplied with each product just before use. 

Assay 20 µl of the gaussia luciferase sample with 50 µl Gaussia luciferase assay reagent. If activity is too high dilute with serum-free DMEM but use  20 µl total volume per assay using 50 µl of Gaussia luciferase assay reagent.

          Mix well and read in the luminometer.