Firefly Luciferase assay reagent Protocol    

Protocol for use of FLAR assay reagent:

ASSAY IN 96-WELL FORMAT: Add 100 µl of the FLAR-1 reagent to 50 µl or 100 µl of the supernatant culture media from cells grown in multi-well tissue culture dishes. Incubate for at least 10 minutes and read in a lumionometer or micro plate reader.

ASSAY OF LUCIFERASE ACTIVITY IN CELL LYSATES: The FLAR-1 luciferase assay reagents can also be used to measure luciferase activity in prelysed cells. Mix 5-50 µl of luciferase containing sample or cell lysate with 100 µl of the luciferase assay kit (FLAR-1) and read immediately in the luminometer. All assay reagents should be close to room temperature at the time of assay

NOTE: If you need to lyse cells first use the cell lysis reagent from Targeting Systems. Protocol for CLR here.