Cell Lysis reagent Protocol    

Protocol for use of 5x CLR reagent:

Dilute the 5X Cell lysis reagent to 1X with water.  Aspirate supernatant cell culture media.  Add enough cell lysis reagent to cover the cells well (150 µl per well of a 24-well dish, 250 µl per well of a 12-well dish, 800 µl per well of a 6-well dish, 2.5 ml per 10 cm dish.  Keep cells of an orbital shaker for 15-20 mins .  Use 5-20 µl of the lysate for assay.

The cell lysis buffer from Targeting Systems is compatible with assay reagents for firefly,  renilla or Gaussia luciferase