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Sequence and Features of pGLUC KDEL Basic-1 Vector
Size: 4932 bases |
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Sequence, plasmid map and features of pGLUC Basic-1 aagatcaagggggccggtggtgacaaagacgaactgtaggcggccgctcgagc
Size- 4932 bases gacggatcgggagatcttggaattctgcagatatcctcgagcccaagctt ggtaccgagctcggatccagccacc atgggagtcaaagttctgtttgccc tgatctgcatcgctgtggccgaggccaagcccaccgagaacaacgaagac ttcaacatcgtggccgtggccagcaacttcgcgaccacggatctcgatgc tgaccgcgggaagttgcccggcaagaagctgccgctggaggtgctcaaag agatggaagccaatgcccggaaagctggctgcaccaggggctgtctgatc tgcctgtcccacatcaagtgcacgcccaagatgaagaagttcatcccagg acgctgccacacctacgaaggcgacaaagagtccgcacagggcggcatag gcgaggcgatcgtcgacattcctgagattcctgggttcaaggacttggag cccatggagcagttcatcgcacaggtcgatctgtgtgtggactgcacaac tggctgcctcaaagggcttgccaacgtgcagtgttctgacctgctcaaga agtggctgccgcaacgctgtgcgacctttgccagcaagatccagggccag gtggacaagatcaagggggccggtggtgacaaagacgaactgtaggcggccgctcgagcatg catctagaaataattcttactgtcatgccaagtaagatgcttttctgtgc tgcaatagcaggcatgctggggatgcggtgggctctatggcttctgaggc ggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcg gcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctaca cttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttct cgccacgttcgccggctttccccgtcaagctctaaatcggggcatccctt tagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgat tagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcg ccctttgacgttggagtccacgttctttaatagtggactcttgttccaaa ctggaacaacactcaaccctatctcggtctattcttttgatttataaggg attttggggatttcggcctattggttaaaaaatgagctgatttaacaaaa atttaacgcgaattaattctgtggaatgtgtgtcagttagggtgtggaaa gtccccaggctccccaggcaggcagaagtatgcaaagcatgcatctcaat tagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaag tatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaa ctccgcccatcccgcccctaactccgcccagttccgcccattctccgccc catggctgactaattttttttatttatgcagaggccgaggccgcctctgc ctctgagctattccagaagtagtgaggaggcttttttggaggcctaggct tttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcaa gagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgc aggttctccggccgcttgggtggagaggctattcggctatgactgggcac aacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcag gggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatga actgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttc cttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctg ctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcc tgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgc ttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgag cgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctgga cgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaagg cgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgc ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactg tggccggctgggtgtggcggaccgctatcaggacatagcgttggctaccc gtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtg ctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgcct tcttgacgagttcttctgagcgggactctggggttcgaaatgaccgacca agcgacgcccaacctgccatcacgagatttcgattccaccgccgccttct atgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatc ctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtt tattgcagcttataatggttacaaataaagcaatagcatcacaaatttca caaataaagcatttttttcactgcattctagttgtggtttgtccaaactc atcaatgtatcttatcatgtctgtataccgtcgacctctagctagagctt ggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctca caattccacacaacatacgagccggaagcataaagtgtaaagcctggggt gcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgc tttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaac gcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctc actgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctca ctcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaa agaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggcc gcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaa aaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagat accaggcgtttccccctggaagctccctcgtgcgctctcctgttccgacc ctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggc gctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttc gctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgc gccttatccggtaactatcgtcttgagtccaacccggtaagacacgactt atcgccactggcagcagccactggtaacaggattagcagagcgaggtatg taggcggtgctacagagttcttgaagtggtggcctaactacggctacact agaaggacagtatttggtatctgcgctctgctgaagccagttaccttcgg aaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcg gtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatct caagaagatcctttgatcttttctacggggtctgacgctcagtggaacga aaactcacgttaagggattttggtcatgagattatcaaaaaggatcttca cctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtata tatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacc tatctcagcgatctgtctatttcgttcatccatagttgcctgactccccg tcgtgtagataactacgatacgggagggcttaccatctggccccagtgct gcaatgataccgcgagacccacgctcaccggctccagatttatcagcaat aaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttat ccgcctccatccagtctattaattgttgccgggaagctagagtaagtagt tcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgt ggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaac gatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagc tccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatc actcatggttatggcagcactgcataattctcttactgtcatgccatccg taagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaa tagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataa taccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgtt cttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcg atgtaacccactcgtgcacccaactgatcttcagcatcttttactttcac cagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagg gaataagggcgacacggaaatgttgaatactcatactcttcctttttcaa tattattgaagcatttatcagggttattgtctcatgagcggatacatatt tgaatgtatttagaaaaataaacaaataggggttccgcgcacatttcccc gaaaagtgccacctgacgtc Features of pGLUC-KDEL- Basic-1 (4920 bp) T7 promoter bases: 20-38 Polylinker bases: 12-63 Gaussia luciferase reporter gene(566 bp) : 76-642- SP6 promoter: 669-686 Synthetic polyadenylation site: 653-697 SV40 promoter bases: 1253-1573 SV40 origin of replication: bases 1352-1437 Neomycin ORF : bases 1609-2403 SV40 PolyA: bases 2458-2830 ColE1 origin: bases 3090-3763, Ampicillin ORF: bases 3908-4768
The plasmid map shown above was constructed with the help of the following link: http://wishart.biology.ualberta.ca/PlasMapper/jsp/displayPlasmidMap.jsp?fileName=plasMap115_1101977745436.jpg&fileFormat=jpg
The CMV promoter is covered by U.S. Patents 5,168,062 and 5,385,839 and its use is permitted for research purposes only. Any other use of the CMV promoter requires a license from the University of Iowa Research Foundation, 214 Technology Innovation Center, Iowa City, IA 52242 Gaussia luciferase is covered by US Patent # 6,232, 107 and IPO patents issued to Prolume Inc. Pittsburgh, PA. This plasmid is being sold for research purposes only. Commercial use will require licenses from Prolume for the Gaussia gene and from the Univ. of Iowa Research foundation for use of the CMV promoter.
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