Size: 4920 bases
 

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gacggatcgggagatcttggaattctgcagatatcctcgagcccaagctt

ggtaccgagctcggatccagccaccatgggagtcaaagttctgtttgccc

tgatctgcatcgctgtggccgaggccaagcccaccgagaacaacgaagac

ttcaacatcgtggccgtggccagcaacttcgcgaccacggatctcgatgc

tgaccgcgggaagttgcccggcaagaagctgccgctggaggtgctcaaag

agatggaagccaatgcccggaaagctggctgcaccaggggctgtctgatc

tgcctgtcccacatcaagtgcacgcccaagatgaagaagttcatcccagg

acgctgccacacctacgaaggcgacaaagagtccgcacagggcggcatag

gcgaggcgatcgtcgacattcctgagattcctgggttcaaggacttggag

cccatggagcagttcatcgcacaggtcgatctgtgtgtggactgcacaac

tggctgcctcaaagggcttgccaacgtgcagtgttctgacctgctcaaga

agtggctgccgcaacgctgtgcgacctttgccagcaagatccagggccag

gtggacaagatcaagggggccggtggtgactaagcggccgctcgagcatg

catctagaaataattcttactgtcatgccaagtaagatgcttttctgtgc

tgcaatagcaggcatgctggggatgcggtgggctctatggcttctgaggc

ggaaagaaccagctggggctctagggggtatccccacgcgccctgtagcg

gcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctaca

cttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttct

cgccacgttcgccggctttccccgtcaagctctaaatcggggcatccctt

tagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgat

tagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcg

ccctttgacgttggagtccacgttctttaatagtggactcttgttccaaa

ctggaacaacactcaaccctatctcggtctattcttttgatttataaggg

attttggggatttcggcctattggttaaaaaatgagctgatttaacaaaa

atttaacgcgaattaattctgtggaatgtgtgtcagttagggtgtggaaa

gtccccaggctccccaggcaggcagaagtatgcaaagcatgcatctcaat

tagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaag

tatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaa

ctccgcccatcccgcccctaactccgcccagttccgcccattctccgccc

catggctgactaattttttttatttatgcagaggccgaggccgcctctgc

ctctgagctattccagaagtagtgaggaggcttttttggaggcctaggct

tttgcaaaaagctcccgggagcttgtatatccattttcggatctgatcaa

gagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgc

aggttctccggccgcttgggtggagaggctattcggctatgactgggcac

aacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcag

gggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatga

actgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttc

cttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctg

ctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcc

tgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgc

ttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgag

cgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctgga

cgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaagg

cgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgc

ttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactg

tggccggctgggtgtggcggaccgctatcaggacatagcgttggctaccc

gtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtg

ctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgcct

tcttgacgagttcttctgagcgggactctggggttcgaaatgaccgacca

agcgacgcccaacctgccatcacgagatttcgattccaccgccgccttct

atgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatc

ctccagcgcggggatctcatgctggagttcttcgcccaccccaacttgtt

tattgcagcttataatggttacaaataaagcaatagcatcacaaatttca

caaataaagcatttttttcactgcattctagttgtggtttgtccaaactc

atcaatgtatcttatcatgtctgtataccgtcgacctctagctagagctt

ggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctca

caattccacacaacatacgagccggaagcataaagtgtaaagcctggggt

gcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgc

tttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaac

gcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctc

actgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctca

ctcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaa

agaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggcc

gcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaa

aaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagat

accaggcgtttccccctggaagctccctcgtgcgctctcctgttccgacc

ctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggc

gctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttc

gctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgc

gccttatccggtaactatcgtcttgagtccaacccggtaagacacgactt

atcgccactggcagcagccactggtaacaggattagcagagcgaggtatg

taggcggtgctacagagttcttgaagtggtggcctaactacggctacact

agaaggacagtatttggtatctgcgctctgctgaagccagttaccttcgg

aaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcg

gtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatct

caagaagatcctttgatcttttctacggggtctgacgctcagtggaacga

aaactcacgttaagggattttggtcatgagattatcaaaaaggatcttca

cctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtata

tatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacc

tatctcagcgatctgtctatttcgttcatccatagttgcctgactccccg

tcgtgtagataactacgatacgggagggcttaccatctggccccagtgct

gcaatgataccgcgagacccacgctcaccggctccagatttatcagcaat

aaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttat

ccgcctccatccagtctattaattgttgccgggaagctagagtaagtagt

tcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgt

ggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaac

gatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagc

tccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatc

actcatggttatggcagcactgcataattctcttactgtcatgccatccg

taagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaa

tagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataa

taccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgtt

cttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcg

atgtaacccactcgtgcacccaactgatcttcagcatcttttactttcac

cagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagg

gaataagggcgacacggaaatgttgaatactcatactcttcctttttcaa

tattattgaagcatttatcagggttattgtctcatgagcggatacatatt

tgaatgtatttagaaaaataaacaaataggggttccgcgcacatttcccc

pGLUC Basic-1  (4920 bp)
T7 promoter bases: 20-38
Polylinker bases: 12-63
Gaussia luciferase gene- 63-653
SP6 promoter: 669-686
Synthetic polyadenylation site: 653-697
SV40 promoter bases: 1253-1573
SV40 origin of replication: bases 1352-1437
Neomycin ORF : bases 1609-2403
SV40 PolyA: bases 2458-2830
ColE1 origin: bases 3090-3763,     Ampicillin ORF: bases 3908-4768

The plasmid map shown above was constructed with the help of the following link:

Xiaoli Dong, Paul Stothard, Ian J. Forsythe, and David S. Wishart "PlasMapper: a web server for drawing and auto-annotating plasmid maps" Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W660-4.