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Sequence and Features of pCMV RedFLUC Vector
Size: 6827 bases |
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1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ccatggaaac agaaagagaa gaaaacgttg tctacggccc actgccattc 961 tacccgatcg aggagggctc tgccggcatc caattgcaca agtacatgca acaatacgcc 1021 aagctcggcg ccatcgcctt cagtaacgcc ctgacaggcg tcgacatcag ctaccagcag 1081 tacttcgaca tcacgtgcag actcgccgag gctatgaaga actacggcat gaagccagaa 1141 ggacacatcg ctctctgtag cgagaactgc gaagagttct tcattcctgt tctggctggt 1201 ctttacatcg gagttacagt cgcgccaact aacgaaattt atacacttag agagctgaac 1261 cacagtctgg ggatagccca acctactatc gtattctcta gcaggaaggg cctgcccaaa 1321 gtgcttgagg tgcagaagac cgtgacttgc atcaaaacca ttgtcatcct ggacagtaag 1381 gtcaacttcg gcggttatga ctgcgtagag accttcatta agaaacacgt cgagctgggc 1441 tttcctgcca cctcatttgt gcccatcgac gtcaaagacc ggaagcacca cattgctctg 1501 cttatgaact cttccggttc cacagggctg cccaaaggag tagagatcac tcacgaggcc 1561 ctggtcacga gattctctca cgctaaggac cctatatacg gcaatcaggt ggccccaggt 1621 accgctatcc tgactgtcgt gcctttccac cacggcttcg gaatgttcac tactttgggc 1681 tactttgcct gcggttaccg gattgtcatg cttactaagt tcgacgagga gcttttcctg 1741 cgcacacttc aggattacaa gtgcactaca gtaatcctgg tgccgacact gttcgcaatt 1801 cttaataggt ctgagctcct tgataagttt gacctctcta acctgactga aatagccagc 1861 ggtggtgctc cacttgccaa ggagatcggc gaggctgttg caagaagatt caacctccca 1921 ggcgtccggc agggatatgg actcaccgag actaccagtg cctttatcat cactcctaag 1981 ggcgacgaca agccgggagc cagcggcaag gtcgtgcctc tgttcaaggt gaagattatt 2041 gacctcgata ccaagaaaac gttgggtgtc aacagacggg gagaaatctg cgtgaaagga 2101 ccatctctta tgttgggata cacgaacaat cctgaagcca ccagagaaac tattgacgag 2161 gaaggctggc tgcacacggg tgacatcggg tactacgacg aggatgagca cttctttata 2221 gtcgaccgcc tgaaatctct cattaagtat aaaggatacc aagtgccacc agctgaactg 2281 gagtctgtgc tcctgcaaca ccctaacatt agagatgctg gtgtggccgg ggttcccgac 2341 agcgaggcag gcgagctgcc tggagccgtc gttgtgatgg aaaagggaaa gacaatgact 2401 gagaaagaaa tcgtagacta tgtaaactcc caggtggtca accacaagcg gctgaggggc 2461 ggcgtgcggt tcgtagatga agtccccaag gggctcacag gaaagatcga cgcgaaagtt 2521 atcagggaga tactcaagaa acctcaagca ggtgggtagt ctagatctag aaataattct 2581 tactgtcatg ccaagtaaga tgcttttctg tgctgcaata gcaggcatgc tggggatgcg 2641 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 2701 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 2761 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 2821 ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt 2881 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 2941 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 3001 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 3061 gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 3121 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 3181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 3241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 3301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 3361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag 3421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 3481 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg 3541 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc 3601 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg 3661 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag 3721 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc 3781 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat 3841 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 3901 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc 3961 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag 4021 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc 4081 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc 4141 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata 4201 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc 4261 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac 4321 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc 4381 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 4441 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 4501 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 4561 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 4621 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 4681 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 4741 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 4801 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 4861 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 4921 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 4981 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 5041 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 5101 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 5161 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 5221 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac 5281 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 5341 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 5401 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 5461 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 5521 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 5581 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 5641 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 5701 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 5761 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 5821 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 5881 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 5941 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 6001 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 6061 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 6121 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 6181 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 6241 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 6301 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 6361 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 6421 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 6481 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 6541 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 6601 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 6661 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 6721 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 6781 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
pCMV-RedFLuc (6827 bp) CMV promoter bases: 209-863 Red luciferase gene: 907-2560 T7 promoter bases: 1827-1845 Polylinker bases: 1852-1870 SP6 promoter: 2576-2593 Synthetic polyadenylation site: 2560-2604 SV40 promoter bases: 3145-3480 SV40 origin of replication: bases: 3259-3344 Neomycin ORF : bases 3516- 4310 SV40 PolyA: bases 4365-4737 ColE1 origin: bases 3934-4607 Ampicillin ORF: bases 4752-5612
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