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Sequence and Features of pCMV GrFLUC Vector
Size: 6827 bases |
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1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
901 gagctcggat ccatggaaac agaaagagaa gaaaacgttg tctacggccc actgccattc
961 tacccgatcg aggagggctc tgccggcatc caattgcaca agtacatgca acaatacgcc
1021 aagctcggcg ccatcgcctt cagtaacgcc ctgacaggcg tcgacatcag ctaccagcag
1081 tacttcgaca tcacgtgcag actcgccgag gctatgaaga actacggcat gaagccagaa
1141 ggacacatcg ctctctgtag cgagaactgc gaagagttct tcattcctgt tctggctggt
1201 ctttacatcg gagttacagt cgcgccaact aacgaaattt atacacttag agagctgaac
1261 cacagtctgg ggatagccca acctactatc gtattctcta gcaggaaggg cctgcccaaa
1321 gtgcttgagg tgcagaagac cgtgacttgc atcaaaacca ttgtcatcct ggacagtaag
1381 gtcaacttcg gcggttatga ctgcgtagag accttcatta agaaacacgt cgagctgggc
1441 tttcctgcca cctcatttgt gcccatcgac gtcaaagacc ggaagcacca cattgctctg
1501 cttatgaact cttccggttc cacagggctg cccaaaggag tagagatcac tcacgaggcc
1561 ctggtcacga gattctctca cgctaaggac cctatatacg gcaatcaggt ggccccaggt
1621 accgctatcc tgactgtcat ccctttccac cacgccttcg gaatgagcac tactttgggc
1681 tactttgcct gcggttaccg gattgtcatg cttactaagt tcgacgagga gcttttcctg
1741 cgcacacttc aggattacaa gtgcactagc gtaatcctgg tgccgacact gttcgcaatt
1801 cttaataggt ctgagctcct tgataagttt gacctctcta acctgactga aatagccagc
1861 ggtggtgctc cacttgccaa ggagatcggc gaggctgttg caagaagatt caacctccca
1921 ggcgtccggc agggatatgg actcaccgag actaccagtg cctttatcat cactcctaag
1981 ggcgacgaca agccgggagc cagcggcaag gtcgtgcctc tgttcaaggt gaagattatt
2041 gacctcgata ccaagaaaac gttgggtgtc aacagacggg gagaaatctg cgtgaaagga
2101 ccatctctta tgttgggata cacgaacaat cctgaagcca ccagagaaac tattgacgag
2161 gaaggctggc tgcacacggg tgacatcggg tactacgacg aggatgagca cttctttata
2221 gtcgaccgcc tgaaatctct cattaagtat aaaggatacc aagtgccacc agctgaactg
2281 gagtctgtgc tcctgcaaca ccctaacatt agagatgctg gtgtggccgg ggttcccgac
2341 agcgaggcag gcgagctgcc tggagccgtc gttgtgatgg aaaagggaaa gacaatgact
2401 gagaaagaaa tcgtagacta tgtaaactcc caggtggtca accacaagcg gctgaggggc
2461 ggcgtgcggt tcgtagatga agtccccaag gggctcacag gaaagatcga cgcgaaagtt
2521 atcagggaga tactcaagaa acctcaagca ggtgggtagt ctagaaataa ttcttactgt
2581 catgccaagt aagatgcttt tctgtgctgc aatagcaggc atgctgggga tgcggtgggc
2641 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
2701 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
2761 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
2821 ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta
2881 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
2941 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
3001 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
3061 ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
3121 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccaggcaggc
3181 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
3241 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
3301 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
3361 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
3421 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
3481 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
3541 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
3601 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
3661 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
3721 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
3781 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
3841 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
3901 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
3961 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
4021 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
4081 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
4141 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
4201 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
4261 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
4321 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
4381 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
4441 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
4501 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
4561 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
4621 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
4681 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
4741 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
4801 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
4861 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
4921 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
4981 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
5041 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
5101 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
5161 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
5221 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
5281 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
5341 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
5401 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
5461 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
5521 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
5581 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
5641 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
5701 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
5761 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
5821 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
5881 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
5941 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
6001 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
6061 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
6121 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
6181 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
6241 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
6301 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
6361 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
6421 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
6481 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
6541 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
6601 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
6661 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
6721 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
6781 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
pCMV-GrFLuc (6827 bp) CMV promoter bases: 209-863 Green emitting firefly luciferase gene: 907-2560 T7 promoter bases: 1827-1845 Polylinker bases: 1852-1870 SP6 promoter: 2576-2593 Synthetic polyadenylation site: 2560-2604 SV40 promoter bases: 3145-3480 SV40 origin of replication: bases: 3259-3344 Neomycin ORF : bases 3516- 4310 SV40 PolyA: bases 4365-4737 ColE1 origin: bases 3934-4607 Ampicillin ORF: bases 4752-5612
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