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Sequence and Features of pCMV GLUC-1 Vector
Size: 5764 bases |
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gacggatcgggagatctcccgatcccctatggtcgactctcagtacaatc tgctctgatgccgcatagttaagccagtatctgctccctgcttgtgtgtt ggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaag gcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcg ctgcttcgcgatgtacgggccagatatacgcgttgacattgattattgac tagttattaatagtaatcaattacggggtcattagttcatagcccatata tggagttccgcgttacataacttacggtaaatggcccgcctggctgaccg cccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagt aacgccaatagggactttccattgacgtcaatgggtggactatttacggt aaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccc cctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta catgaccttatgggactttcctacttggcagtacatctacgtattagtca tcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtgga tagcggtttgactcacggggatttccaagtctccaccccattgacgtcaa tgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgta acaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggag gtctatataagcagagctctctggctaactagagaacccactgcttactg gcttatcgaaattaatacgactcactatagggagacccaagcttggtacc gagctcggatccagccaccatgggagtcaaagttctgtttgccctgatct gcatcgctgtggccgaggccaagcccaccgagaacaacgaagacttcaac atcgtggccgtggccagcaacttcgcgaccacggatctcgatgctgaccg cgggaagttgcccggcaagaagctgccgctggaggtgctcaaagagatgg aagccaatgcccggaaagctggctgcaccaggggctgtctgatctgcctg tcccacatcaagtgcacgcccaagatgaagaagttcatcccaggacgctg ccacacctacgaaggcgacaaagagtccgcacagggcggcataggcgagg cgatcgtcgacattcctgagattcctgggttcaaggacttggagcccatg gagcagttcatcgcacaggtcgatctgtgtgtggactgcacaactggctg cctcaaagggcttgccaacgtgcagtgttctgacctgctcaagaagtggc tgccgcaacgctgtgcgacctttgccagcaagatccagggccaggtggac aagatcaagggggccggtggtgactaagcggccgctcgagcatgcatcta gaaataattcttactgtcatgccaagtaagatgcttttctgtgctgcaat agcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaag aaccagctggggctctagggggtatccccacgcgccctgtagcggcgcat taagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgcc agcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccac gttcgccggctttccccgtcaagctctaaatcggggcatccctttagggt tccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggt gatggttcacgtagtgggccatcgccctgatagacggtttttcgcccttt gacgttggagtccacgttctttaatagtggactcttgttccaaactggaa caacactcaaccctatctcggtctattcttttgatttataagggattttg gggatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaa cgcgaattaattctgtggaatgtgtgtcagttagggtgtggaaagtcccc aggctccccaggcaggcagaagtatgcaaagcatgcatctcaattagtca gcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgca aagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgc ccatcccgcccctaactccgcccagttccgcccattctccgccccatggc tgactaattttttttatttatgcagaggccgaggccgcctctgcctctga gctattccagaagtagtgaggaggcttttttggaggcctaggcttttgca aaaagctcccgggagcttgtatatccattttcggatctgatcaagagaca ggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttc tccggccgcttgggtggagaggctattcggctatgactgggcacaacaga caatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgc ccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgca ggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcg cagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattg ggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccga gaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatc cggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagca cgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaaga gcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgca tgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccg aatatcatggtggaaaatggccgcttttctggattcatcgactgtggccg gctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgata ttgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttac ggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttga cgagttcttctgagcgggactctggggttcgaaatgaccgaccaagcgac gcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaa ggttgggcttcggaatcgttttccgggacgccggctggatgatcctccag cgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgc agcttataatggttacaaataaagcaatagcatcacaaatttcacaaata aagcatttttttcactgcattctagttgtggtttgtccaaactcatcaat gtatcttatcatgtctgtataccgtcgacctctagctagagcttggcgta atcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattc cacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaa tgagtgagctaactcacattaattgcgttgcgctcactgcccgctttcca gtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcgg ggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgac tcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaa ggcggtaatacggttatccacagaatcaggggataacgcaggaaagaaca tgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttg ctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcg acgctcaagtcagaggtggcgaaacccgacaggactataaagataccagg cgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccg cttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttc tcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctcca agctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgcctta tccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgcc actggcagcagccactggtaacaggattagcagagcgaggtatgtaggcg gtgctacagagttcttgaagtggtggcctaactacggctacactagaagg acagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaag agttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtt tttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaa gatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactc acgttaagggattttggtcatgagattatcaaaaaggatcttcacctaga tccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgag taaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctc agcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgt agataactacgatacgggagggcttaccatctggccccagtgctgcaatg ataccgcgagacccacgctcaccggctccagatttatcagcaataaacca gccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcct ccatccagtctattaattgttgccgggaagctagagtaagtagttcgcca gttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtc acgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaa ggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttc ggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcat ggttatggcagcactgcataattctcttactgtcatgccatccgtaagat gcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgt atgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgc gccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcgg ggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaa cccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgt ttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataa gggcgacacggaaatgttgaatactcatactcttcctttttcaatattat tgaagcatttatcagggttattgtctcatgagcggatacatatttgaatg tatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaag tgccacctgacgtc
pCMV-GLUC-1 (5764 bp) CMV promoter bases: 209-863 Gaussia luciferase gene: 907-1497 T7 promoter bases: 864-882 Polylinker bases: 889-907 SP6 promoter: 1513-1530 Synthetic polyadenylation site: 1497-1541 SV40 promoter bases: 2082-2417 SV40 origin of replication: bases 2196-2281 Neomycin ORF : bases 2453-3247 SV40 PolyA: bases 3302-3674 ColE1 origin: bases 3934-4607 Ampicillin ORF: bases 4752-5612
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